An antibody shouldn t be one of the variables in your experiment.
Cell signaling anti lamin.
Lamin a and c are cleaved by caspases into large 41 50 kda and small 28 kda fragments which can be used as markers for apoptosis 4 5.
Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
The expected protein mass is 74 1 kda but there are 6 reported isoforms.
Phosphorylation of lamin a c at ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens.
Actin filaments were labeled by dylight 554 phalloidin 13054 cell signaling technologies danvers ma usa.
During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.
2000 j struct biol 129 313 23.
Incubate membrane with anti rabbit igg hrp linked antibody 7074 at 1 2000 and anti biotin hrp linked antibody 7075 at 1 1000 1 3000 to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.
Type b lamins consist of lamin b1 and b2 encoded by separate genes 6 8.
Proceed with detection section d.
Type b lamins consist of lamin b1 and b2 encoded by separate genes 6 8.
Lamin a is a reported synonym of the human protein lamin a c encoded by the gene lmna.
It is listed to be a member of the intermediate filament family.
The cells were fixed in 2 paraformaldehyde for 10 min at room temperature.
Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation.
2000 j struct biol 129 313 23.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
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The full protein is reported to be 664 amino acid residues in length.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
Lamin b1 is also cleaved by caspases during apoptosis 9.
Lamin b1 is also cleaved by caspases during apoptosis 9.
In humans this protein is encoded by the gene lmna.
Lamin a and c are cleaved by caspases into large 41 50 kda and small 28 kda fragments which can be used as markers for apoptosis 4 5.
The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a.
Anti lamin a c antibodies are available from several suppliers.
Anti lamin a antibodies are readily available from several suppliers.
